![]() ![]() ![]() 3Īcquire Mountain Key from room through door to the left of the Throne Room Acquire Fire Book I, and use it immediately. Enter center door, then right door to reach the heal room. Take either path back into the 4th floor of the castle. On Third Floor, right door leads to room with Star Key. On Second Floor, right door leads to Lightning Gate. Spirit, then Somersault -> Cyclone (^ v ^ ^ ^) works best. Do not use Gimard magic more than once per fight. Skeletons are very difficult to run from. Note: Run from fights with 1x Drake Ghost or 2x Gimard. Item 7: Door of Light – Drake Castle Courtyard Fight 1-2 battles as needed to reach level 2. Item 5: Point Card (in Drawer after touching World Map for 1st time) Item 4: Mei’s Pendant (from Mei when leaving) I recommend using Charging Scorch (v > ^) I would reset if you don’t get it by the second try. (Must talk to Mei, take her to your house or item 4 is permanently missed)įight Gimard and get the Gimard spell (55% chance). Item 1: Healing Leaf x3 (Shopkeep in Rim Elm, after Mist, pre-Meta – permanently missed after getting Meta!) Night: Talk to Village Elder to advance plot On the first turn, attack with a single command to save a fraction of a second.Īt dusk, go to Vahn’s house & talk to Val to advance to night. Vahn’s House – more mashing Tetsu training. When Mei talks about the hunter’s clothes, second option is faster. Unlike many games, there is no 1 character / frame limitation on text. Still, it’s wise to cross-check for completeness’s sake (I did, and you should too because who wants to do a run this long and then miss an item halfway through…)ĭefault Names are fastest. With that said, the order in which the items are listed on that guide isn’t the best way to pick up all the items, so I’ll be getting them in a different order. I recommend 42, but 41 is probably safe.Ī full checklist of all items, with some pictures of where they are, can also be found here. We’ll also have Vahn with Crimson Book to get a lot of EXP, since he’ll need lots of levels for Master Course. Since you’ll be in the best shape to take them out, and absorb rates are highest at 元 (and you’ll have Ivory Books), we’ll get the bare minimum number of Seru throughout the game, then grind for them all in Bio-Castle. The good news for Seru absorption is that NO Seru are permanently missable they all appear in Bio-Castle at 元. Then there’s Lapis, Master Course, and Earth Egg, the unholy trinity of the run.Īll treasures tracked by the in-game tracker on the end screen are collected (260/260) We just have to pick up a lot of stuff and absorb all the Seru on all characters. All the stuff I wrote about tips and tricks for any% continues to hold true in this category. This is a long run, and not for the faint-of-heart. I highly suggest starting off with any% or any% no slots. First, to the poor soul who got suckered in to running this category, you have my sympathies. ![]()
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![]() Someone who stood up and said NO to the CCP. This means leveraging the law to our advantage and thinking creatively to counter the influence and economic extortion of the Chinese Communist Party.Īnd we had that, before 2020. Washington needs to wage “lawfare” just as aggressively as Beijing is presently doing. We need to advocate for international norms and standards that conform to liberal western rules, and not the authoritarian proclivities of the Chinese Communist Party.Īs if we can get that with the current “administration”, and beijing joe and commie kamala! We need to call out the economic extortion of the Chinese Communist Party vocally and often in international fora and multilateral engagements. ![]() We need to offer competitive financing and loan guarantees for countries wishing to rebuild or redevelop their infrastructure – guarantees not loaded with quid-pro-quo requirements or onerous terms that will ensure their failure. What has been missing is leadership from Washington. General Secretary Xi Jinping would make a great stand-in for Tony Soprano if they decided to reboot the series and set it in Beijing.īut I heard too many fools WANT communism. In case after case, we see the Chinese Communist Party offering overly generous terms to countries in need of development and assistance, only to saddle them with unrealistic payments while corrupting the political systems of the countries in question and ending up with the assets in the end. In Sri Lanka, too, the Chinese Communist Party, through the Belt and Road Initiative managed to get Columbo to hand over a port and some 15,000 acres for a 99-year lease after the government couldn’t make payments on the development project.Īny why is the legacy media not reporting on this? In Ecuador, a dam project that was ostensibly intended to help lift the country out of poverty led to a national scandal with shoddy workmanship, unmet promises, bribes and the Chinese Communist Party effectively owning the dam in question as Quito could not keep up with the payments. A member of city council was allegedly offered upwards of $360,000 to drop her opposition to the agreement. In Africa, allegations of bribery followed Huawei as it attempted to secure an exclusive contract to build a 5G network in Namibia. The economic extortion isn’t limited to Australia. …they are indicative of a much more alarming trend in the behavior of the Chinese Communist Party, which amounts to economic extortion and coercion in support of its geopolitical aims. To fight China’s economic extortion, take a page from the Cold War ![]() Win for the CCP, a win for its aggressive expansion and control.Ī win for those who want communists to control key infrastructure in other nations.Ī lose for Montenegro is a WIN for the CCP. “This cooperation is mutually beneficial and win-win,” said China’s Montenegrin embassy in a statement last month. “It would take at least 22-25,000 vehicles a day for the highway to pay off,” civil engineer Ivan Kekovic told AFP, roughly four times the number he could envisage on the busiest stretch. The current government has admitted revenue from tolls will not even cover the road’s annual maintenance, estimated at 77 million euros ($94 million). If Montenegro cannot pay, it faces arbitration in Beijing and could be forced to give up control of key infrastructure, according to a copy of the contract seen by AFP.Ĭhina has been widely criticised for saddling small countries with unmanageable debt as part of its global Belt and Road Initiative.Ĭritics worry that it will use financial leverage to boost its political power, in what they dub “debt-trap diplomacy”.īut Chinese officials have strenuously denied any ulterior motive to the investment in Montenegro and the wider region. ![]() It is unclear where the money will come from or how Montenegro - a country with a GDP of 4.9 billion euros - will repay its existing debt to China. The government has already burnt through $944 million in Chinese loans to complete the first stretch of road, just 41 kilometres (25 miles)…Īlmost 130 kilometres still needs to be built at a likely cost of at least one billion euros ($1.2 billion). I recall news stories warning countries of accepting the CCPs massive building projects.Ĭhinese workers have spent six years carving tunnels through solid rock and raising concrete pillars above gorges and canyons, but the road in effect goes nowhere. Two sleek new roads vanish into mountain tunnels high above a sleepy Montenegrin village, the unlikely endpoint of a billion-dollar project bankrolled by China that is threatening to derail the tiny country’s economy. Montenegro learns true cost of China-backed $1 bn road to nowhere ![]() ![]() dfbdf on Audio Damage – AD054 Other Desert Cities 1.0.9 (VST, VST3, AAX, AU).thesterling on Big Fish Audio – Stupid Crush: K-Pop Construction Kits (MiDi, Acidized WAV).Stupid Shit on Drumforge – DrumShotz Andrew Wade (WAV).Yoj on Sample Magic – Resampled Soul Selections (WAV).Admin on Sample Magic – Resampled Soul Selections (WAV).Admin on Audio Damage – AD054 Other Desert Cities 1.0.9 (VST, VST3, AAX, AU).Greg on FabFilter – Total Bundle 2023.3 – CE-V.R (VST, VST3, AAX).leekhazaa on StudiolinkedVST OvO RnB (Kontakt).Sergio Barreda on Audio Assault – Duality Bass Studio 1.2.5 (STANDALONE, VST, VST3, AAX, AU).So Me on Sonic Projects – OP-X PRO-II 1.2.6 (VSTi, VSTi3, AUi).So Me on Sonic Projects OP-X PRO II v1.0 (VST).John on Applied Acoustics Systems – Lounge Lizard EP-4 v4.4.3 (SAL, VSTi, VST3i, AAX, AU).lalal on Image-Line – FL Studio Producer Edition 20.vale on Image-Line – FL Studio Producer Edition 20.Admin on Sonic Projects OP-X PRO II v1.0 (VST).Admin on Sonic Projects – OP-X PRO-II 1.2.6 (VSTi, VSTi3, AUi).Admin on Audio Assault – Duality Bass Studio 1.2.5 (STANDALONE, VST, VST3, AAX, AU).Admin on StudiolinkedVST OvO RnB (Kontakt).Toni on Tone2 – Electra 3 v3.2.1 – R2R (STANDALONE, VSTi, VST3i).Portable, No Install & SymLink Installer.Apple – Logic Pro X 10.7.8 – TNT 300 views.1 – Drum Sample Pack (Ableton Project, WAV) 300 views Production Music Live – Deep Premium Vol.Pulsar Modular – P440 Sweet Spot v1.1.1 – R2R (VST3, AAX) 300 views.Skillshare – How to become a Synth Runner – Synthesizer Sound Design Class with Arturia Pigments (TUTORIAL) 300 views.KORG – Collection 4 03.2023 – R2R + RET (STANDALONE, VSTi, VSTi3, AAX) 300 views.Oeksound – Soothe2 v.1.1.2 (VST, VST3, AAX) 300 views.Xfer Records – Serum v1.36b3 (SAL, VSTi, VSTi3, AAX) 500 views.Synapse Audio – The Legend 1.5.0 – R2R (VSTi, VSTi3, AAX) 500 views.Ableton – Live 11 Suite 11.3.2 600 views.– 1337x.to – demonoid.ph – – Įiosis - E☭EESSER Pan Drums FiDef Groove FST Arturia - Synth Collection WA Production - Redhead Roman EDM Essentials For SYLENTH1 Total Samples MIA Laboratories - 925 COMPRESSOR SonicWire ADSR LetiMix Rises Loopmasters - UK Drill TBProAudio - gEQ12 NKC FXpansion - BFD Eldorado Sampletank Bowed Piano The Cargo Cult ![]() Work around Virtual Guitarist file descriptor leak bug.įix plugin manager crashes when non-AU plugins placed in AU plugins folder. All your 32-bit only plug-ins will again re-appear in the plug-ins menu as they always did.įix rare crashes when closing Virtual Guitarist and some other VSTGUI based plugins. No special re-wiring or routing is required. Older Logic 9 sessions will completely load into Logic Pro X, including all presets, parameters and automation. Previous projects that were created in Logic 9 or earlier that include 32-bit only plug-ins will not fully open in Logic Pro X.ģ2 Lives is a new application, capable of generating 64-bit Audio Units versions of your loved and hard-earned 32-bit legacy plug-ins, helping you to cross over to the new Logic Pro X smoothly and transparently. Moving forward with new technologies, the new Logic Pro X is a modern, 64-bit only application, which means that older 32-bit plug-ins will no longer work in Logic Pro X. In July 2013, Apple introduced Logic Pro X, the next-generation version of its professional audio software, bringing many new features and improvements and an updated user interface. ![]() Macintosh HD /Library/Application Support/Sound Radixģ2 Lives is a 32-bit to 64-bit Audio-Units plug-ins adapter, and is compatible with virtually all 64-bit Audio-Units host applications. ![]() Keep in mind if you have the previous version, you have created a hidden folder in Application Support (remove). ![]() Packaged……: Bergelmir Release-Type.: Audio ![]() ![]() ![]() Sticky ends from different BaeGI sites may not be compatible. Sticky ends from different StyI sites may not be compatible. Sticky ends from different BtgI sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different AflIII sites may not be compatible.īsrGI is typically used at 37☌, but is even more active at 60☌. ![]() ![]() ![]() Get properties for file Hospital.cfg, and untick Read-only attribute. Copy folder Hosp from the game CD into location you want. Il est vivement conseillé d'installer Theme Hospital sous DOS si vous ne voulez pas avoir de bug au cours du jeu et bien voila enfin un patch combiné à un crack NO CD qui enlève les bugs du jeu et permet de jouer sans le CD (Vous pouvez le télécharger en cliquant ici). Theme Hospital is somewhat newer for a DOS game, so you'll need to increase the speed of the DOSBox emulation to get it running a bit better. In DOSBox, type in the following commands: mount c d:/games/dos (Or the path to whatever your folder happens to be) c: dir cd/themeh~1 dir hospital 5. Theme Hospital No CD Cracks Crack for Version 1.0 ENG Crack for Version. No-CD Patch Theme Hospital ANY VERSION MULTI If you have problems using a trainer in combination with Windows Vista, 7 or 8 then make sure to run the. Game Fix / Crack: Theme Hospital v1.1 NoDVD NoCD | MegaGames. I might see if I still have a copy of the original. AFAIK all budget versions have the game's 1.1 patch applied by default. What sort of version was it aimed at? It won't work with my budget copy because neither the DOS or Windows version have an exe with the amount of bytes in size that the crack is expecting. When this happens use the original EXE to play online, else you could find yourself banned from the game! When using Fixed Files make sure to use a Firewall which controls outgoing traffic, as some. Some No-CD/Fixed EXE files work fine in Single Player mode but are detected to be modified when trying to play online. Cd problem: just copy your Hospital game to c:\Hospital. zip file and put all the contents into the newly created HOSPITAL folder then should work no problems. to everyone having issues with the no cd open the folder called hospital (the one in lower case) which was in the. Theme Hospital PC Game 1997 Overview Theme Hospital is developed under the banner of Bullfrog Productions for Microsoft Windows. Theme Hospital 1997 is a business simulation game. Theme Hospital Free Download PC Game setup in single direct link for windows. Other Articles That You May Be Interested In: Edward Teller Biography Dubbed the father of the H-Bomb, Teller played a big role in the Manhattan Project. ![]() Return to the No CD Cracks for PC Games Section.Insert the CD into your CD-ROM drive, which I will assume is called. Download and install DOSBox from Create a folder to store DOS games, called C:/DOS (or whatever you like, but this guide will assume you're using C:/DOS). How to get Theme Hospital Working on Windows Vista or XP. So, when you open dosbox without starting the game, you should be able to cd to the INSTALL_PATH as written in HOSPITAL.CFG and end up at a directory with the. On Theme Hospital the CD required to play message is what appears when it cannot find all the game files. Home✿ixes»PC»Theme Hospital»Maciozo no CD Theme Hospital All. Your name (Login to post using username, leave blank to post as Anonymous). ![]() ![]() ![]() The population in Region Halland aged 65 years and above and free from AF will be randomized into two halves, creating a general cohort and an RPM cohort. Then in the next step for the prospective study, applying the RPM on the RPM cohort, the at-risk group will be extracted for randomization step, using the recommended cut-off value. Specifically, we are looking to calibrate the intercept (α) for the logistic regression where we already have the 13 odd ratios for the 13 risk factors from the original study. We will include patients ≥45 years of age at index date, which is the first date of an AF diagnosis recorded in the observation period and a random pseudo index date during the observation period for the control group, to follow the original study. ![]() To calibrate the BMS/Pfizer risk prediction model (RPM), we will extract two cohorts retrospectively: the AF cohort with an AF diagnosis (patients with a record of incident AF diagnosis between January 1, 2016, and Decemas an observation period), and the control cohort without any AF diagnosis in their history. ![]() Study Population: Residents in Region Halland age 65 and above.ĭata Collection Methods: Electronic Health Records from Region Halland and 14-days continuous ECG recording using an ECG patch. Objective(s): To compare the yield of atrial fibrillation (AF) using 14-days continuous ECG in a population aged ≥ 65 years with an increased risk for AF incidence according to the risk prediction model compared with standard of care in Region Halland. Condition or diseaseĭiagnostic Test: Risk prediction model Diagnostic Test: 14-days continuous ECG monitoring The main objective of this study is to test the hypothesis that AF screening with 14-days continuous ECG monitoring in high-risk individuals identified with a risk prediction model is more effective than routine care in identifying patients with undetected AF.Įffectively detecting AF among patients with risk factors for ischaemic stroke has the potential to decrease mortality and morbidity, stroke burden and costs for the society as a whole. Screening is recommended in European guidelines, however the most suitable population and the most suitable device for AF detection remain to be defined. It is important to find patients with AF and additional risk factors for stroke in order to initiate oral anticoagulation treatment, which can reduce the risk of an ischaemic stroke by 60-70%. As AF is often asymptomatic there are many undetected cases. AF increases the risk of ischaemic stroke fivefold and accounts for almost one-third of all strokes. Why Should I Register and Submit Results?Ītrial fibrillation (AF) is the most common clinical arrhythmia and the prevalence increases with age. ![]() ![]() a city park permitting scheme requiring advance application for gatherings of more than 50 individuals, in Thomas v.Community for Creative Non-Violence (1984) a National Park Service regulation prohibiting camping (including as a form of protest) in certain federal parks, determined in Clark v.Examples of content-neutral laws that have been upheldĮxamples deemed content neutral by the Supreme Court include: is whether the government has adopted a regulation of speech because of disagreement with the message it conveys.”Ĭontent-neutral laws must regulate speech without reference to the speech’s substance, be narrowly tailored, and leave open alternative avenues of expression. Rock Against Racism (1989) that “the principal inquiry in determining content neutrality, in speech cases generally. ![]() Reviewing courts often label a law as content based or content neutral by determining whether the government passed the law in order to suppress expression. Often, the distinction is determines the outcome of a case, as many content-based laws are struck down, but many content-neutral laws survive judicial inquiry. Content neutral laws are often found to be constitutional Such laws generally regulate only the time, place, and manner of speech in contrast to content-based laws, which regulate speech based on content. This distinction is important in First Amendment cases because courts hold content-based laws to strict scrutiny - the highest form of judicial review - while holding content-neutral laws only to intermediate, or mid-level, scrutiny. (AP Photo/Fred Prouser, used with permission from the Associated Press)Ĭontent neutral refers to laws that apply to all expression without regard to the substance or message of the expression. ![]() In this photo, a member of the Hare Krishna Religious sect solicits funds at the Pennsylvania Farm show in Harrisburg, Pennsylvania, Jan. International Society for Krishna Consciousness (1981) after members of the Krishna sect alleged it restricted the First Amendment exercise of religion. An example of a content neutral law is a Minnesota rule prohibiting the sale or distribution of any merchandise, including printed material, in parks. Content neutral refers to laws that apply to all expression without regard to the substance or message of the expression. ![]() ![]() He said action had now been taken to restrict the use of diplomatic/official passports.Īsked whether the Gampola narcotics ring could have been involved with a Canadian passport holder now in Sri Lankan custody regarding an abortive bid to smuggle in 36 kilos of heroin in early March last year, the police spokesman said that the Police Narcotics Bureau (PNB) was continuing investigations. "I was told how bundles of money had been distributed among the people." The Police Spokesman said that the investigation was continuing.Īppearing live on Sirasa political programme on Thursday night, DM Perera said that he had an opportunity to organise the Opposition campaign at the Gampola electorate at a recent election. Responding to Deputy Foreign Minister Perera, Police spokesman SSP Ajith Rohana acknowledged that the police hadn’t recorded the then Premier Jayaratne’s statement, though the police had questioned his son, Central Provincial Council member Anuradha Lanka Jayaratne as regards his involvement. Jayaratne’s Office in a major heroin smuggling operation involving Pakistanis to a conclusion. The Deputy Minister said the police hadn’t been able to bring an investigation into the alleged use of then Prime Minister D. He said that anyone having a diplomatic/official passport, unless he or she was entitled to it should be subject to scrutiny. ![]() The DM said that it would be of pivotal importance to identify those who had abused their diplomatic/official passports and as well as their political masters. The Kalutara District MP emphasised the responsibility on the part of the police to check with the Immigration and Emigration to establish the identities of those who had received diplomatic passports through political influence. Responding to a query by The Island, Perera claimed that they had used the VIP channel for that purpose for years. Perera yesterday alleged that some of those who had received diplomatic/official passports during the UPFA administration were believed to have exploited the facility to smuggle in narcotics through the Bandaranaike International Airport. Link between Gampola gang and Canadian Lankan held for smuggling 36 kgs of heroin to be probedĭeputy Foreign Minister Ajith P.
![]() Meyers then spied his quarterback, Mac Jones, near the middle of the field and uncorked a ball backward that stayed in the air for what seemed like an eternity.Ĭhandler Jones, after missing the tackle on Stevenson, was again pushed to the ground by Patriots right guard Mike Onwenu but ended up in the right place at the right time for Las Vegas. The Error-maculate Interception? How else to describe Jakobi Meyers' inexplicable attempt to keep hope alive for the Patriots, rather than simply go down and play for overtime?īecause with three seconds on the clock of a tie game and New England on its 45-yard line, Patriots running back Rhamondre Stevenson burst up the middle - through Jones' grasp - for a 23-yard gain before he pitched to Meyers along the right sideline. "I don't know what they're going to call this one," Raiders coach Josh McDaniels said, "but it will probably have a nickname here shortly." And after this one, they'll reimagine the Heidi Game.īecause what Raiders defensive end Chandler Jones pulled off - a 48-yard walk-off fumble return after he snatched an ill-fated cross-field lateral to give the Raiders a 30-24 victory - was something entirely different. But they'll smile at the Sea of Hands and embrace the Holy Roller. The Raiders and their fans will forget the Tuck Rule Game and ignore the Immaculate Reception. "Have you ever seen an ending like that before?" "It's a new house of thrills," Davis told ESPN with a grin. LAS VEGAS - Mark Davis traipsed across the Las Vegas Raiders' plush locker room in Allegiant Stadium late Sunday afternoon with a dazed look on his face after the latest chapter in the wild and woolly history of the franchise he owns had unfolded. NFL, Las Vegas Raiders, New England Patriots Raiders stun Pats: 'Have you ever seen an ending like that?' This should be one of the highest-scoring offenses in the league.You have reached a degraded version of because you're using an unsupported version of Internet Explorer.įor a complete experience, please upgrade or use a supported browser They are loaded with talented pass catchers and an All-Pro running back in Josh Jacobs. It won’t be long before he is a Pro Bowl tight end in the NFL.Īs long as Jimmy Garoppolo can stay healthy, the Raiders should be among the most exciting offenses in the NFL. ![]() He is a reliable target in the middle of the field and is the most productive tight end in Notre Dame history. Despite trading Darren Waller to the Giants, the Raiders were able to replace him with Notre Dame’s Michael Mayer, considered one of the best tight ends in a deep 2023 draft class, to go along with Austin Hooper.Īs PFF mentioned, the addition of Michael Mayer should also help make this offense that much more explosive. With free-agent signing Jakobi Meyers joining Davante Adams and Hunter Renfrow, this receiver room suddenly becomes very potent. ![]() Here is a snippet of their thoughts on the unit: In a recent article by Pro Football Focus, they wrote about the receiving group of the Raiders heading into the year. ![]() It’s easily their biggest strength heading into the 2023 season. Needless to say, it’s one of the best positional groups in the NFL as the Raiders are loaded with talented pass catchers. Then, they traded for Davante Adams in 2022 and have now added Jakobi Meyers in free agency. They just signed Hunter Renfrow to a big contract extension after his Pro Bowl season in 2021. The Raiders have invested a ton into their wide receivers over the last two off-seasons. ![]() ![]() Received: JAccepted: DecemPublished: January 7, 2019Ĭopyright: © 2019 Ren et al. Copenhaver, The University of North Carolina at Chapel Hill, UNITED STATES (2019) BRASSINOSTEROID-SIGNALING KINASE 3, a plasma membrane-associated scaffold protein involved in early brassinosteroid signaling. Together, our findings elucidate the function of BSK3 in BR signaling and plant growth and development, and provide new insights into early BR signaling mechanisms.Ĭitation: Ren H, Willige BC, Jaillais Y, Geng S, Park MY, Gray WM, et al. ![]() BSK3 activates BR signaling via upregulating BSU1 transcript levels, and BIN2 phosphorylation of BSK3 influences BSK3 interactions with other signaling components, revealing new layers of regulation in BR signaling. BSK3 is important for BR-mediated root growth, shoot growth, and organ separation. Our genetic and biochemical studies suggest that BSK3 may function as a scaffold protein to regulate BR signaling. Here, we focus on BSK3 and investigate its function in BR signaling and plant growth and development. However, the functions of these receptor-like cytoplasmic kinases in the BR signaling pathway are poorly understood. Discovered in 2008, the BRASSINOSTEROID-SIGNALING KINASE (BSK) protein family is composed of twelve members. Brassinosteroids (BRs) are steroid hormones that regulate numerous growth and developmental processes throughout the plant life cycle. Steroid hormones exist in both animals and plants. The results of our studies provide new insights into early BR signaling mechanisms. Together, our findings suggest that BSK3 may function as a scaffold protein to regulate BR signaling. BSK3 is broadly expressed and plays an important role in BR-mediated root growth, shoot growth, and organ separation. Furthermore, we find that BSK3 upregulates BSU1 transcript and protein levels to activate BR signaling. BIN2 phosphorylation of BSK3 enhances BSK3/BSK3 homodimer and BSK3/BSK1 heterodimer formation, BSK3/BRI1 interaction, and BSK3/BSU1 interaction. BSK3 directly interacts with the BSK family proteins (BSK3 and BSK1), BRI1 receptor kinase, BSU1 phosphatase, and BIN2 kinase. Our genetic studies indicate that kinase dead BSK3 K86R protein partially rescues the bsk3-1 mutant phenotypes. Interestingly, the effects of BSK3 on BR responses are dose-dependent, depending on its protein levels. The N-terminal kinase domain is crucial for BSK3 function, and the C-terminal three tandem TPR motifs contribute to BSK3/BSK3 homodimer and BSK3/BSK1 heterodimer formation. We find that BSK3 is anchored to the plasma membrane via N-myristoylation, which is required for its function in BR signaling. ![]() We therefore investigated the function of BSK3, a receptor-like cytoplasmic kinase, in BR signaling and plant growth and development. Through forward genetics, we identified five semi-dominant mutations in the BSK3 gene causing BSK3 loss-of-function and decreased BR responses. The BR signaling pathway has been studied in some detail, however, the functions of the BRASSINOSTEROID-SIGNALING KINASE (BSK) family proteins in the pathway have remained elusive. Brassinosteroids (BRs) are steroid hormones essential for plant growth and development. ![]() |
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